Flush the system using one of the cleaning mixtures as described in the cleaning solution table of the controlling contamination in uplc ms and hplc ms sytems guide 715001307.
Waters hplc system cleaning procedure.
2 0 scope this procedure is applicable for of all high performance liquid chromatography installed in quality control laboratory.
Ultrapure water is water that has been purified through a system that targets contami nants detrimental to uplc ms and hplc ms systems.
Reverse osmosis to remove most contaminants b.
Technical assistant 3 2 checking.
Disconnect the column and attach a union and flow directly to waste.
No injections are needed with that.
Water flush to clean acid from the system.
To clean the system.
Label bottles correctly with bottle content and filling date expiry date.
Use only hplc grade solvents and water filtered through 0 2.
When preparing a system to run your method it is always important to prime up the system to ensure you get fresh solvents in all of the required lines.
To clearly lay down the procedure for cleaning.
The water flushes are only needed to remove the previous solvent from the system.
To clean the system.
Water flush to clear customer s mobile phases from the system.
Place a b c and d seal wash and needle wash lines into a clean.
It is necessary to restore the system flow path to a near new level of cleanliness note.
Remove all solvent filters.
Objective to lay down the procedure for operation of hplc waters alliance 2695 separation module scope to ensure that the instrument performs satisfactory and gives accurate and reproducible data.
Cleaning mixtures for lc table in the controlling contamination in ultraperformance lc ms and hplc ms systems document p n 715001307.
3 0 responsibility 3 1 doing.
Maintaining your hplc uhplc uplc systems.
When working with any lc system setting it up and storing it is very important.
Responsibility quality control officer executive.
Cleaning decontamination columns general guidelines flow cells general.
Controlling contamination in lc ms and hplc ms systems 715001307.
The purification process should include all of the following steps.
Ion exchange to remove any remaining ions.
This sop shall be applicable for the hplc system waters alliances 2690 2695 separation modules.
Residues or contaminations may block filters or capillaries.
Use solvent inlet filters to protect the system from incoming particles.
Executive manager 4 0 accountability head of the department 5 0 procedure 5 1 for system cleaning.
